Unconditional backbone generation
Generate one backbone with fixed seed 0, 15–50 diffusion steps, and no target conditioning.
Start this methodGenerate a bounded unconditional backbone with RFdiffusion
Generate one deterministic 20–50-residue protein backbone with the RFdiffusion Base model.
Choose an approach
Scientific approaches
Select the analysis that best matches your scientific question. Each approach opens with its relevant inputs and controls.
Prepare
Bounded generation parameters
No source Dataset is required. Supply a target residue count and diffusion-step count inside the supported range.
- Length from 20 through 50 residues
- Diffusion steps from 15 through 50
- Exactly one design with deterministic seed 0
Length
20 residuesDiffusion steps
15How the analysis starts
Choose a Project, open a compatible Dataset, then select the rows you want to analyze. Ubi will open this method with the compatible controls and column mappings ready to review.
Configure the scientific method
These controls appear in the Dataset analysis panel, where values can be checked against the actual input before the run starts.
Backbone length
20–50 residues
Choose the exact length encoded in the unconditional contig.
Diffusion schedule
15–50 steps
More steps cost more CPU time; each analysis produces one design.
Generation model
RFdiffusion Base · deterministic seed 0
Keep the model and seed fixed when comparing diffusion schedules.
Review results as scientific outputs
Results open with the figures, structures, sequences, and metrics needed to answer the scientific question. Downloadable files remain available for downstream analysis.
How to interpret the result
- The output is an unconditional backbone proposal, not a sequence, binder, motif scaffold, or experimentally validated structure.
- Review geometry and add independently qualified sequence-design and validation steps before experimental use.
- Compare repeats only when checkpoint, source trees, length, step count, seed, and runtime image are identical.
1
Predicted backbone structure
Open the PDB and inspect residue count, backbone atoms, and coordinates.
2
Generation settings
Review backbone length, diffusion steps, seed, and model alongside the structure.
Continue your analysis
Use a complementary method on the same Project data.
Method scope and limitations
- This tool supports unconditional Base-model generation only; target-conditioned and symmetry modes are not available.
- Backbone generation may take tens of seconds to minutes.
