Analysis method

Compare predicted nanobody polyreactivity

Apply both published Harvey et al. CDR-only logistic models to heavy-chain nanobody variable domains and retain the exact model and numbering evidence.

Method details

Input checks

  • Canonical 20-amino-acid alphabet only
  • 80–200 residues per sequence and at most 100 rows
  • AbNumber must classify the variable domain as heavy chain under IMGT numbering
  • CDR lengths must fit the fixed published one-hot feature profiles

Expected results

  • Relative decision-score comparison — Review original-FACS and deep-FACS scores side by side; higher values indicate lower predicted polyreactivity.
  • CDR and biochemical evidence — Inspect aligned CDRs, CDR lengths, isoelectric point, Kyte–Doolittle hydrophobicity, and N-glycosylation motif flags.
  • Immutable execution evidence — Retain request, model-file, model-bundle, native-result, and derived Dataset lineage.
Example result
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Ubi Biologics