Humanization method
Humanize and assess antibody variable domains
Generate humanized variants or measure exact OAS repertoire 9-mer prevalence, then inspect the sequence-level evidence before selecting candidates.
Method details
Input checks
- 40–180 unambiguous amino acids per variable domain
- Heavy, kappa, or lambda chain type supplied per row
- Up to 16 sequences in one analysis
Expected results
- Parental-to-variant residue map — Review numbered substitutions by framework or CDR region.
- Humanization score comparison — Compare assigned residue scores before and after humanization.
- Germline, identity, and repertoire summary — For humanization, inspect V/J assignments and mutations; for OASis, inspect identity, percentile, and human/non-human 9-mer counts.
- Low-prevalence peptide map — Locate each low-prevalence overlapping 9-mer and see which numbered residues it affects.
Example results
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