Sapiens humanization
Model-guided variable-domain humanization with exact residue-level comparisons.
Start this methodHumanization method
Run BioPhi online
Humanize and assess antibody variable domains
Generate humanized variants or measure exact OAS repertoire 9-mer prevalence, then inspect the sequence-level evidence before selecting candidates.
Choose an approach
Scientific approaches
Select the analysis that best matches your scientific question. Each approach opens with its relevant inputs and controls.
Human-germline CDR grafting
CDR grafting with optional Vernier restoration and Sapiens refinement.
Start this methodOASis repertoire humanness
Exact overlapping 9-mer prevalence, OASis identity, therapeutic-mAb percentile, and residue-linked rare-peptide evidence.
Start this methodPrepare
Antibody variable-domain sequences
Start from a protein or annotated-sequence Dataset with one sequence per row and an H, K, or L chain assignment.
- 40–180 unambiguous amino acids per variable domain
- Heavy, kappa, or lambda chain type supplied per row
- Up to 16 sequences in one analysis
Sequence column
sequenceChain type
H, K, or LHow the analysis starts
Choose a Project, open a compatible Dataset, then select the rows you want to analyze. Ubi will open this method with the compatible controls and column mappings ready to review.
Configure the scientific method
These controls appear in the Dataset analysis panel, where values can be checked against the actual input before the run starts.
Analysis
Humanization or OASis scoring
Choose sequence design when proposing variants, or repertoire scoring when comparing existing candidates.
Humanization method
Sapiens or CDR grafting
Choose model-guided substitutions or graft CDRs onto a human germline framework.
Numbering and CDR definition
Kabat, IMGT, or Chothia
Use the convention that matches your sequence review and downstream assays.
Residue treatment
1–5 passes, optional Vernier restoration
Control optimization depth and whether parental Vernier residues are restored.
Review results as scientific outputs
Results open with the figures, structures, sequences, and metrics needed to answer the scientific question. Downloadable files remain available for downstream analysis.
How to interpret the result
- Sequence identity reports how much of the parental variable domain is retained; a higher value means fewer proposed substitutions.
- Compare parental and humanized assigned scores residue by residue. They are model scores, not direct measurements of immunogenicity.
- Use numbered mutations and framework/CDR labels to identify changes that need structural, binding, or assay review.
- OASis identity is the fraction of overlapping 9-mers meeting the selected human-subject prevalence threshold; it is a repertoire-prevalence proxy, not a direct immunogenicity probability.
- Use the peptide and residue maps to localize low-prevalence sequence segments before deciding whether a substitution is biologically acceptable.
1
Parental-to-variant residue map
Review numbered substitutions by framework or CDR region.
2
Humanization score comparison
Compare assigned residue scores before and after humanization.
3
Germline, identity, and repertoire summary
For humanization, inspect V/J assignments and mutations; for OASis, inspect identity, percentile, and human/non-human 9-mer counts.
4
Low-prevalence peptide map
Locate each low-prevalence overlapping 9-mer and see which numbered residues it affects.
Continue your analysis
Use a complementary method on the same Project data.
Method scope and limitations
- OASis uses the official OASis_9mers_v1 database and exact BioPhi denominator semantics; it does not substitute a germline similarity or Sapiens score.
- Results are computational proposals and should be reviewed alongside binding and developability evidence.
