Numbering and CDR definition
IMGT, Chothia, Kabat, or AHo
Choose residue coordinates and optional liability-region context.
Annotate antibody sequences
Number antibody variable domains, assign V/J germlines, label FR/CDR regions, and inspect sequence liabilities in one analysis.
Prepare
Antibody variable-domain sequences
Use a protein or annotated-sequence Dataset with one variable-domain sequence per row.
- Unambiguous protein sequence in the selected column
- One frozen Selection or Dataset Version
- Up to 1,000 rows and 500 residues per row
Sequence column
sequenceReference species
human, mouse, rabbit, alpaca, or unknownHow the analysis starts
Choose a Project, open a compatible Dataset, then select the rows you want to analyze. Ubi will open this method with the compatible controls and column mappings ready to review.
Configure the scientific method
These controls appear in the Dataset analysis panel, where values can be checked against the actual input before the run starts.
Germline species
Explicit species database
Select the reference population used for V/J assignment.
Annotation options
Liabilities and optional CDR3 masking
Control liability annotation and the scope of the model score.
Review results as scientific outputs
Results open with the figures, structures, sequences, and metrics needed to answer the scientific question. Downloadable files remain available for downstream analysis.
How to interpret the result
- Germline identity is reference similarity, not a direct immunogenicity measurement.
- Review liability calls in their numbered FR/CDR context and against assay evidence.
- Keep the reference species and numbering scheme fixed when comparing candidates.
1
Chain and region annotation
Inspect numbered FR/CDR residues and chain classification.
2
V/J germline assignment
Review reference genes and sequence identity scores.
3
Sequence liabilities
Locate reported sequence liabilities with one-based source positions.
Continue your analysis
Use a complementary method on the same Project data.
Method scope and limitations
- This method annotates protein sequences; DNA translation and sequence validation are separate analyses.
- Results are computational annotations and do not replace experimental developability assessment.
