OncologyImmuno-oncologyImmunology & inflammation QSPTCE/BsAb

CD3×5T4 In Vitro Activation and Cytotoxicity QSP

Fixed-effect in vitro model linking CD3×5T4 immune-synapse formation to T-cell activation, differentiation, and delayed tumor-cell killing.

Therapeutic: DuoBody-CD3x5T4, bsIgG1-CD3x5T4
Modality: Bispecific antibody / T-cell engager
Target: CD3, 5T4
Disease: In vitro solid-tumor cell-line assay (not disease-specific)
Model type: QSP, TCE/BsAb

Complete model workspace

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Parameters: 33
States: 17
Equations: 17
Derived outputs: 18

Explore this model

Choose a starting point to view its result. Adjust key model inputs when you want to explore a different outcome.

Starting configuration

MDA-MB-468 untreated, E:T 4:1

Null-control culture with 16,000 tumor cells and 64,000 resting T cells, matching the referenced assay setup.

Starting result

This result reflects the starting settings. Run your changes to update it.

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Starting configuration

Time: 0–72 hour; step 1

Input	Default	Available range
CD3-arm 3D dissociation constant	683 nM	341.5–1,025 nM
Trimer threshold for half-maximal activation	2.13 molecule/cell	1.065–3.195 molecule/cell
Maximum T-cell activation rate	0.0106 1/hr	0.0053–0.0159 1/hr
Aggregate trimer threshold for half-maximal killing	7,098 molecule	3,549–1.06e+4 molecule
Maximum per-effector tumor killing rate	1.30e-5 1/(hour*cell)	6.50e-6–1.95e-5 1/(hour*cell)

Expected readouts

Immune-synapse trimers per T cell (trimers/T cell) · Activated or effector T cells (%) · Effector T cells (cells/well) · Tumor-cell cytotoxicity (%) · Viable and apoptotic tumor cells (cells/well)

Adjustable model parameters

This public explorer exposes 5 curated parameters. Sign in to edit all 33 declared model parameters.

CD3-arm 3D dissociation constant DuoBody-CD3x5T4 value; the high-affinity bsIgG1 variant is 16 nM. Values between the endpoints are exploratory and values outside them are outside this runtime contract. nMTrimer threshold for half-maximal activation MDA-MB-468 cell-line group 3 value. molecule/cellMaximum T-cell activation rate Upper rate of trimer-dependent resting-to-activated T-cell conversion in the fixed-effect model. 1/hrAggregate trimer threshold for half-maximal killing Aggregate trimer load across effector T cells producing half-maximal per-effector killing drive. molecule

More key parameters (1)

Maximum per-effector tumor killing rate MDA-MB-468 cell-line group 4 and no-donor/donor group 2 value. 1/(hour*cell)

How the model represents the biology

Links per-cell CD3×5T4 trimer formation to resting-to-activated-to-effector T-cell dynamics and three-stage apoptotic tumor-cell loss across drug affinity, concentration, antigen density, and effector-to-target ratio.

Scenario outputs

The full model defines 18 derived outputs. These are the curated readouts used across the scenario plots above.

Immune-synapse trimers per T cell trimers/T cell

Read together with total effector-cell abundance: a high per-cell trimer signal does not by itself imply a large aggregate killing drive.
Activated or effector T cells %

An in vitro state fraction, not a clinical response rate or a cytokine-release measurement.
Effector T cells cells/well

This reflects the source differentiation mechanism and should not be interpreted as tumor infiltration in vivo.
Tumor-cell cytotoxicity %

A model-derived in vitro cytotoxicity readout; it is not a clinical tumor-response endpoint.
Viable and apoptotic tumor cells cells/well

Dead tumor cells are excluded after leaving the final apoptosis stage.

Found a scientific issue? Email helpdesk@unibiointelligence.com with model ID yuan_2026_cd3x5t4_in_vitro_qsp.

Research use only — not for patient-specific prediction or dosing advice.

CD3×5T4 In Vitro Activation and Cytotoxicity QSP

Explore this model

MDA-MB-468 untreated, E:T 4:1

Starting result

Starting configuration

Adjustable model parameters

How the model represents the biology

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